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Gym Rework and Raid Battles A comprehensive guide on how to trigger EX Raids

Full guide on how to trigger an EX Raid in your city, based on S2 cells, gym eligibility and expected player and raiding activity. After months of Exclusive Raids we finally have answers to a number of questions:

Over the past few weeks, we worked hard to read and collect player feedback from all around the world and this guide is the culmination of that work.

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What do we know about EX Raids so far?

Don’t worry if you don’t understand all the terms right now, that’s OK, we explain each term in it’s own section we’ll try to explain every step of the “triggering” thoroughly.

Let the triggering begin!

Level 12 S2 cells only host one EX raid sponsored placed in a park related OpenStreetMap tag

Step #1: Discovering your local S2 cell geometry

EX Raids can only occur once inside each S2 cell that passes over your local area. S2 cells are a simple mathematical way to describe a sphere’s geometry. S2 cells come in different sizes, often denoted as levels, and they are often used by developers to create zones on spherical objects, like the planet Earth.

Niantic uses them to control weather, create gyms, define Pokémon catch / hatch locations, etc… For this step we’ll be using the Level 12 S2 cell geometry.

A company named Sidewalk Labs created an incredible tool that draws Level 12 cells over a Google Map. Head over to their Cheap Sale Store Buy Cheap New Styles Faye linen minidress Three Graces London Cheapest JcH6fEW1n1
and do the following:

Now that you can see your local S2 cells, remember that an EX Raid can only occur once per cell per cycle. Raiding 100 gyms inside one cell doesn’t benefit you as much as raiding a dozen of eligible gyms in a higher number of cells.

In the next step, we’ll see how to identify eligible gyms.

min level 12, max level 12, max cells 10 Use one of the drawing tools

Step #2: Finding eligible gyms

Since November 11 2016, the only gyms that are eligible for hosting EX Raids are as follows:

For this guide, we’re focusing on the gyms located in parks and similar areas . The easiest way to check what the game considers to be a park, is to use a tool called Overpass Turbo. Overpass Turbo is a free online tool that can plot locations of various OpenStreetMap tags.

To find eligible gyms, we will use Overpass Turbo to plot out areas that are tagged as parks (or related tags) on OpenStreetMap:

To start, navigate to DRESSES Short dresses Becamp;bridge Cheap How Much Many Colors 5KFH4h
:

Thecomplex query we use was written by Tntnnbltn and it uses multiple OSM tags to draw areas which are eligible and confirmed as EX Host areas. Only gyms that fall into these areas are eligible for EX Raids .

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Gene set enrichment networks. (A) Network displaying gene sets enriched in hypermistranslating strain T1FH relative to strain T0FH evolved in the presence of fluconazole. (B) Network displaying gene sets enriched in strain T1 evolved in the presence of a high fluconazole concentration (T1FH) relative to strain T1 evolved without the drug (T1NF). (C) Network displaying gene sets enriched in hypermistranslating strain T1 evolved without the drug (T1NF) versus nonevolved strain T1. Edge width represents the number of shared genes, node size represents the number of annotated genes, and node color represents the enrichment based on the number of upregulated (red) or downregulated (blue) genes in the set. The 10 genes ranked highest in each gene set are shown. Download FIG S5, PDF file, 0.9 MB .
Genes expressed differentially in T1FH and T1NF and functional analysis. (Top) Venn diagram in which each colored area represents a set of DEGs (differential expression threshold of ≥3.0, adjusted value of <0.01). Only one gene, , is significantly upregulated in T1FH and downregulated in T1NF. The top two GO terms for each condition are shown. (Bottom) GO BP categories enriched below a value of 0.01 in all of the expression sets shown in the Venn diagram. Download Cheap How Much Carmen Eyelash Lace Deep Brazilian Black Asos Curve Outlet Fast Delivery Cheap 100% Original Great Deals Cheap Online Outlet Great Deals iGhOvpJ
.
FIG 3

Gene expression heatmap. Depicted are DEGs with a 4-log-fold change for at least one comparison and a false discovery rate-adjusted value of <1e−18.

Mating and cell surface alterations. WGS and CGH also showed that T1 lost the MTLa locus (including the nonsex genes PAP1 , PIKa , and OBPa ) during evolution in fluconazole ( Fig. 2 and Cheap Sale Choice DRESSES Kneelength dresses ISABEL GARCIA Discount High Quality Cheap Sale Clearance Store uE48n2lI
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) and increased the copy number of several genes on Chr7 involved in mating, transport, and translation, including orf19.6583, orf19.7038, FLU1 , WHI3 , TOM70 , NBP2 , YCF1 , and GCN2 ( Table 1 ).

(A) Flow cytometry profiles of two T1FH clones. Red, diploid control; blue, sample. The T1FH profile indicates a mixed population of diploids, tetraploids, and some cells with greater ploidy. (B) Confirmation of loss of the locus in T1FH by targeted PCR. Colony PCR products of the (red) and (blue) genes visualized on an agarose gel. is absent from all tested clones of the evolved and highly resistant strain T1FH. For the sequences of the primers used, see I. Miranda, R. Rocha, M. C. Santos, D. D. Mateus, G. R. Moura, L. Carreto, and M. A. Santos, PLoS One 2:e996, 2007. Download FIG S7, EPS file, 0.4 MB .
FIG 4

Highlighted features of quickly evolving and highly resistant strain T1. Mistranslation appears to affect the phosphatidylinositol signal transduction pathways that control several cell cycle events, cell membrane and cell wall remodeling, as well as protein synthesis and turnover, via , most of which are known to enhance drug resistance. The main features of highly drug-resistant strain T1FH are depicted, i.e., loss of and the nonsex gene ; phosphatidylinositol signaling and downstream targets such as DNA repair, cell wall remodeling, and mating; -mediated modulation of protein synthesis; drug efflux pumps; and bud neck formation.

View this table:
TABLE 1

Copy number variation in T1FH

In addition to those genome alterations, in T1FH, we observed a 6.5-fold increase in the expression of MFalpha , the alpha factor mating pheromone gene, and a gene set enrichment of mating-specific terms like bud neck and ascospore formation, pheromone-dependent transcription involved in conjugation with cellular fusion, and terms related to virulence, like adhesion to a host and immune response ( Fig. 3 ; Sale Perfect Muscle tank top Green Nimble Activewear Outlet With Paypal Order Online SlI798WN
). These gene expression alterations in T1FH are possibly related to loss of the MTLa locus and/or the LOH of Chr5 ( 28 30 ).

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